Anti-Ferritin Light Chain Polyclonal Antibody is a Rabbit antibody targeting Ferritin Light Chain. Anti-Ferritin Light Chain Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,ELISA.

IgG

Human

1. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Ferritin Light Chain) Polyclonal Antibody, Unconjugated (TMAB-05967-1) at 1: 2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.
2. Measured by its binding ability in a indirect ELISA. Immobilized Human FTL, His Tag at 2 μg/mL (100 μL/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.5 ng/mL

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

Recombinant Protein: human Ferritin Light Chain

Human

FTL

Ferritin light chain

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.