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Anti-TSPAN1 Antibody (4J370)
🥰Excellent
Catalog No. TMAY-02464
Anti-TSPAN1 Antibody (4J370) is a Rabbit antibody targeting TSPAN1. Anti-TSPAN1 Antibody (4J370) can be used in ELISA,IHC-P,FCM.
Anti-TSPAN1 Antibody (4J370)
Anti-TSPAN1 Antibody (4J370)
🥰Excellent
Catalog No. TMAY-02464
Anti-TSPAN1 Antibody (4J370) is a Rabbit antibody targeting TSPAN1. Anti-TSPAN1 Antibody (4J370) can be used in ELISA,IHC-P,FCM.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-TSPAN1 Antibody (4J370) is a Rabbit antibody targeting TSPAN1. Anti-TSPAN1 Antibody (4J370) can be used in ELISA,IHC-P,FCM. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 4J370 |
| Reactivity | Human |
| Verified Activity | 1. Immunochemical staining of human TSPAN1 in human rectal cancer with rabbit monoclonal antibody (1:10000, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human TSPAN1 in human prostate with rabbit monoclonal antibody (1:10000, formalin-fixed paraffin embedded sections). 3. Flow cytometric analysis of Human TSPAN1 expression on SW480 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human TSPAN1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Application | ELISA,IHC-P,FCM |
| Recommended Dose | ELISA: 1:5000-1:10000; IHC-P: 1:5000-1:20000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human TSPAN1 (rh TSPAN1; TMPY-03936; O60635; Tyr110-Asn211). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | TSPAN1 belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins have four hydrophobic domains, intracellular N- and C-termini and two extracellular domains. Tetraspanins act as scaffolding proteins, anchoring multiple proteins to one area of the cell membrane. They also mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TSPAN1 interacts with human thiamine transporter-1 (hTHTR-1). HTHTR-1 contributes to intestinal thiamine uptake, and its function is regulated at both the transcriptional and posttranscriptional levels. TSPAN1 and hTHTR-1 colocalize in human intestinal epithelial HuTu-8 cells. Coexpression of TSPAN1 in these cells led to a significant decrease in the rate of degradation of hTHTR-1 compared with cells expressing the hTHTR-1 alone; in fact the half-life of the TSPAN1 protein was twice longer in the former cell type compared with the latter cell type. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human TSPAN1 Protein (TMPY-03936) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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