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Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc) V2 is expressed in HEK293 Cells. The accession number is Q16790.

| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 5 μg | $70 | 7-10 days | 7-10 days | |
| 10 μg | $109 | 7-10 days | 7-10 days | |
| 20 μg | $182 | 7-10 days | 7-10 days | |
| 50 μg | $357 | 7-10 days | 7-10 days | |
| 100 μg | $617 | 7-10 days | 7-10 days | |
| 200 μg | $1,070 | 7-10 days | 7-10 days | |
| 500 μg | $2,230 | 7-10 days | 7-10 days | |
| 1 mg | $3,910 | 7-10 days | 7-10 days |
| Biological Activity | Measured by its esterase activity. The specific activity is > 50 pmol/min/µg (QC Test). Immobilized Human CA9, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). |
| Description | Carbonic Anhydrase 9 Protein, Human, Recombinant (hFc) V2 is expressed in HEK293 Cells. The accession number is Q16790. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-hFc |
| Accession Number | Q16790 |
| Synonyms | MN,Carbonic Anhydrase IX,CAIX,CA9,CA 9 |
| Construction | Gln38-Asp414 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE;
> 95% as determined by HPLC |
| Molecular Weight | 66.91 kDa (Predicted); 70-80 kDa (Due to glycosylation) |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| Research Background | CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways. |
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