Immobilized Human TREM2, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TREM2 Antibody, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA (QC Test).
Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay (Biacore T200).

TREM-2 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is Q9NZC2-1.

Human

HEK293 Cells

triggering receptor expressed on myeloid cells 2,Trem2c,Trem2b,Trem2a,TREM-2

His19-Ser174

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2  consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.