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Anti-CREB1 Antibody (9J76) is an antibody targeting CREB1. Anti-CREB1 Antibody (9J76) can be used in ELISA, WB, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, FCM Reactivity: Human, Mouse, Rat |
Ig Type | Rabbit IgG |
Clone | 9J76 |
Reactivity | Human, Mouse, Rat |
Verified Activity | 1. Western Blot -Positive WB detected in: K562 whole cell lysate, THP-1 whole cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, -All lanes: CREB antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 36 kDa -Observed band size: 36 kDa 2. Overlay Peak curve showing Jurkat cells stained with TMAH-00298 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, FCM |
Recommended Dose | WB:1:500-1:2000; FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human CREB1 |
Antigen Species | Human |
Gene ID | 1385 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling, Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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