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Anti-RELB Antibody (2K488)
😃Good
Catalog No. TMAH-01037
Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Anti-RELB Antibody (2K488) is an antibody targeting RELB. Anti-RELB Antibody (2K488) can be used in ELISA, WB, IF, FC.
Anti-RELB Antibody (2K488)
Anti-RELB Antibody (2K488)
😃Good
Catalog No. TMAH-01037Alias v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Anti-RELB Antibody (2K488) is an antibody targeting RELB. Anti-RELB Antibody (2K488) can be used in ELISA, WB, IF, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-RELB Antibody (2K488) is an antibody targeting RELB. Anti-RELB Antibody (2K488) can be used in ELISA, WB, IF, FC. |
| Alias | v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3 |
| Ig Type | Rabbit IgG |
| Clone | 2K488 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, THP-1 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate -All lanes: NF-kB p65 antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 70 kDa -Observed band size: 70 kDa 2. Immunofluorescence staining of PC-3 with TMAH-01037 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 496-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 3. Overlay Peak curve showing Hela cells stained with TMAH-01037 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, IF, FC |
| Recommended Dose | WB:1:500-1:2000; IF:1:50-1:200; FC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells. The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human RELA |
| Antigen Species | Human |
| Gene ID | 5970 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cardiovascular, Cell biology, Metabolism, Microbiology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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