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Anti-NF-kB p65 Antibody (2K488) (Synonyms: v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NF...)

Name: Anti-NF-kB p65 Antibody (2K488)
Brand: TargetMol
SKU: TMAH-01037
Rating: 4.5 (1 reviews)

Catalog No. TMAH-01037 Copy Product Info

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Anti-NF-kB p65 Antibody (2K488) is an antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (2K488) can be used in ELISA,FCM,IF,WB.

Anti-NF-kB p65 Antibody (2K488)

Copy Product Info

🥰Excellent

Catalog No. TMAH-01037

Synonyms v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3

Anti-NF-kB p65 Antibody (2K488) is an antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (2K488) can be used in ELISA,FCM,IF,WB.

Anti-NF-kB p65 Antibody
(2K488)

Pack Size	Price	USA Stock	Global Stock	Quantity
50 μL	$209	7-10 days	7-10 days
100 μL	$347	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Product Introduction

Bioactivity

Description	Anti-NF-kB p65 Antibody (2K488) is an antibody targeting NF-kB p65. Anti-NF-kB p65 Antibody (2K488) can be used in ELISA,FCM,IF,WB.
Synonyms	v-rel avian reticuloendotheliosis viral oncogene homolog A, p65, NFKB3
Ig Type	Rabbit IgG
Clone	2K488
Reactivity	Human
Verified Activity	1. Western Blot -Positive WB detected in: Hela whole cell lysate, THP-1 whole cell lysate, MCF7 whole cell lysate, A549 whole cell lysate -All lanes: NF-kB p65 antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 70 kDa -Observed band size: 70 kDa 2. Immunofluorescence staining of PC-3 with TMAH-01037 at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 496-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). 3. Overlay Peak curve showing Hela cells stained with TMAH-01037 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/110^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application	ELISA FCM IF WB
Recommended Dose	WB:1:500-1:2000; IF:1:50-1:200; FCM:1:50-1:200.
Antibody Type	Monoclonal
Subcellular Localization	Nucleus. Cytoplasm.
Construction	Recombinant Antibody
Purification	Affinity-chromatography
Appearance	Liquid
Formulation	Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research Background	NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells. The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression. Key transcription factor regulating the IFN response during SARS-CoV-2 infection.

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	A synthetic peptide: Human RELA
Antigen Species	Human
Gene Name	RELA
Gene ID	5970
Uniprot ID	Q04206
Biology Area	Epigenetics and Nuclear Signaling, Neuroscience, Cancer, Cardiovascular, Cell biology, Metabolism, Microbiology, Signal transduction

Chemical Properties

Stability & Storage

Stability & Storage	Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
Transport	Shipping with blue ice.

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Keywords

NFKB-3NFKB 3

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