Antibody Type: Mouse Monoclonal

Application: ELISA, WB, ICC, IF, FCM

Reactivity: Human, Mouse, Rat

IgG1

1M448

Human, Mouse, Rat

1. Western Blot
-Positive WB detected in: Rat brain tissue, Mouse brain tissue
-All lanes: NANOG antibody at 1:500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 35, 33 kDa
-Observed band size: 46, 42 kDa
2. Western Blot
-Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate
-All lanes: NANOG antibody at 1:500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 35, 33 kDa
-Observed band size: 46, 40 kDa
3. Immunocytochemistry analysis of TMAH-00803 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. Immunocytochemistry analysis of TMAH-00803 diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
5. Immunofluorescence staining of Hela cells with TMAH-00803 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
6. Immunofluorescence staining of Ntera-2 cells with TMAH-00803 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
7. Overlay histogram showing Hela cells stained with TMAH-00803 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
8. Overlay histogram showing MCF-7 cells stained with TMAH-00803 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
9. Overlay histogram showing Ntera-2 cells stained with TMAH-00803 (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, ICC, IF, FCM

Monoclonal

Unconjugated

Recombinant Protein: Human Homeobox Protein NANOG Protein (1-305AA)

Human

79923

Stem Cells