Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-HSP90AA1 Antibody (3D288)
😃Good
Catalog No. TMAH-00571
Anti-HSP90AA1 Antibody (3D288) is an antibody targeting HSP90AA1. Anti-HSP90AA1 Antibody (3D288) can be used in ELISA, IHC, IF.
Anti-HSP90AA1 Antibody (3D288)
Anti-HSP90AA1 Antibody (3D288)
😃Good
Catalog No. TMAH-00571
Anti-HSP90AA1 Antibody (3D288) is an antibody targeting HSP90AA1. Anti-HSP90AA1 Antibody (3D288) can be used in ELISA, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
Bulk & Custom
Add to Cart
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-HSP90AA1 Antibody (3D288) is an antibody targeting HSP90AA1. Anti-HSP90AA1 Antibody (3D288) can be used in ELISA, IHC, IF. |
| Ig Type | Rabbit IgG |
| Clone | 3D288 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00571 diluted at 1:87.5 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 2. IHC image of TMAH-00571 diluted at 1:87.5 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunofluorescence staining of Hela cells with TMAH-00571 at 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, IHC, IF |
| Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Melanosome. Cell membrane. Mitochondrion. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle. Plays a critical role in mitochondrial import, delivers preproteins to the mitochondrial import receptor TOMM70. Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues(PubMed:25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation. Mediates the association of TOMM70 with IRF3 or TBK1 in mitochodria outer membrane which promotes host antiviral response. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human HSP90AA1 |
| Antigen Species | Human |
| Gene ID | 3320 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Keywords
Renal carcinoma antigen NY-REN-38Lipopolysaccharide-associated protein 2 (LAP-2;LPS-associated protein 2)LAP-2LAP2HSPCAHSPC1HSPC 1HSP90AA1HSP90AHSP90 AA1HSP86HSP 86Heat shock protein HSP 90-αHeat shock protein HSP 90-alphaHeat shock protein HSP 90-aHeat shock protein family C member 1Heat shock 86 kDa (HSP 86;HSP86)
Preview
Related Tags: buy Anti-HSP90AA1 Antibody (3D288) | purchase Anti-HSP90AA1 Antibody (3D288) | Anti-HSP90AA1 Antibody (3D288) cost | order Anti-HSP90AA1 Antibody (3D288)
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.