Anti-AKT1/2/3 Antibody (9A675) is an antibody targeting AKT1/2/3. Anti-AKT1/2/3 Antibody (9A675) can be used in ELISA, WB, IHC, FC.

Rabbit IgG

9A675

Human

1. Western Blot
-Positive WB detected in: SH-SY5Y whole cell lysate
-All lanes: CD44 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 56 kDa
-Observed band size: 56 kDa
2. IHC image of TMAH-00048 diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.
3. Overlay Peak curve showing Jurkat cells stained with TMAH-00048 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

WB:1:500-1:2000; IHC:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

A synthesized peptide derived from Human AKT1/2/3 Antibody

Human

Epigenetics and Nuclear Signaling, Cancer, Metabolism, Signal transduction