Anti-SLC5A1 Polyclonal Antibody is a Rabbit antibody targeting SLC5A1. Anti-SLC5A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat

1. Sample:
Lane 1: Mouse Kidney Lysates
Lane 2: Human 293T cell Lysates
Lane 3: Human HepG2 cell Lysates
Primary: Anti-SLC5A1 (TMAB-01725) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 73 kDa
Observed band size: 70 kDa
2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min
Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min
Blocking buffer (normal goat serum) at 37°C for 20 min
Incubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated (TMAB-01725) 1:200, overnight at
4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (TMAB-01725) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthetic peptide: human SGLT1

Human

SLC5A1

Sodium/glucose cotransporter 1

Metabolism of carbohydrates,Carbohydrate metabolism,Cancer,Channels

Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.