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Anti-SLC5A1 Polyclonal Antibody is a Rabbit antibody targeting SLC5A1. Anti-SLC5A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Anti-SLC5A1 Polyclonal Antibody is a Rabbit antibody targeting SLC5A1. Anti-SLC5A1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Alias | Solute carrier family 5(sodium/glucose cotransporter) member 1, Solute carrier family 5 member 1, Sodium/glucose cotransporter 1, Sodium glucose cotransporter 1, SGLT1, SGLT 1, NAGT, Na(+)/glucose cotransporter 1, Human Na+/glucose cotransporter 1, High affinity sodium glucose cotransporter 1, High affinity sodium glucose cotransporter, GLT-1, GLT1, D22S675 |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Sample: Lane 1: Mouse Kidney Lysates Lane 2: Human 293T cell Lysates Lane 3: Human HepG2 cell Lysates Primary: Anti-SLC5A1 (TMAB-01725) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 70 kDa 2. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min Blocking buffer (normal goat serum) at 37°C for 20 min Incubation: Anti-SGLT1 Polyclonal Antibody, Unconjugated (TMAB-01725) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 3. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (SGLT1) Polyclonal Antibody, Unconjugated (TMAB-01725) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Membrane; Multi-pass membrane protein. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
Immunogen | KLH conjugated synthetic peptide: human SGLT1 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Metabolism of carbohydrates,Carbohydrate metabolism,Cancer,Channels |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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