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Anti-FHIT Antibody (5C583)

😃Good
Catalog No. TMAY-02732
Alias fragile histidine triad, FRA3B, AP3Aase

Anti-FHIT Antibody (5C583) is a Rabbit antibody targeting FHIT. Anti-FHIT Antibody (5C583) can be used in WB,ELISA,ICC/IF.

Anti-FHIT Antibody (5C583)

Anti-FHIT Antibody (5C583)

😃Good
Catalog No. TMAY-02732Alias fragile histidine triad, FRA3B, AP3Aase
Anti-FHIT Antibody (5C583) is a Rabbit antibody targeting FHIT. Anti-FHIT Antibody (5C583) can be used in WB,ELISA,ICC/IF.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: WB, ELISA, ICC/IF

Reactivity: Human
Aliasfragile histidine triad, FRA3B, AP3Aase
Ig Type
Rabbit IgG
Clone
5C583
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
2. Anti-FHIT rabbit monoclonal antibody at 1:100 dilution.
-Lane A: HL-60 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:17 kDa.
-Observed band size:20 kDa
Application
WB,ELISA,ICC/IF
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Fragile histidine triad / FHIT (rh Fragile histidine triad / FHIT; TMPY-03398; P49789; Met1-Gln147).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
Research BackgroundFragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Fragile histidine triad / FHIT Protein (TMPY-03398)
Antigen Species
Human
Biology Area
Tumor Suppressors
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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