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Anti-FHIT Antibody (5C583) is a Rabbit antibody targeting FHIT. Anti-FHIT Antibody (5C583) can be used in WB,ELISA,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $270 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, ICC/IF Reactivity: Human |
Alias | fragile histidine triad, FRA3B, AP3Aase |
Ig Type | Rabbit IgG |
Clone | 5C583 |
Reactivity | Human |
Verified Activity | 1. Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). 2. Anti-FHIT rabbit monoclonal antibody at 1:100 dilution. -Lane A: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:17 kDa. -Observed band size:20 kDa |
Application | WB,ELISA,ICC/IF |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Fragile histidine triad / FHIT (rh Fragile histidine triad / FHIT; TMPY-03398; P49789; Met1-Gln147). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
Research Background | Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human Fragile histidine triad / FHIT Protein (TMPY-03398) |
Antigen Species | Human |
Biology Area | Tumor Suppressors |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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