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Anti-GOLPH2/GOLM1 Antibody (6N611)
😃Good
Catalog No. TMAY-02458
Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Anti-GOLPH2/GOLM1 Antibody (6N611) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (6N611) can be used in WB,ELISA,ICC/IF.
Anti-GOLPH2/GOLM1 Antibody (6N611)
Anti-GOLPH2/GOLM1 Antibody (6N611)
😃Good
Catalog No. TMAY-02458Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Anti-GOLPH2/GOLM1 Antibody (6N611) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (6N611) can be used in WB,ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, ICC/IF Reactivity: Human |
| Alias | PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3 |
| Ig Type | Mouse IgG1 |
| Clone | 6N611 |
| Reactivity | Human |
| Verified Activity | 1. Anti-GP73 mouse monoclonal antibody at 1:500 dilution. -Lane A: 293T Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:45 kDa. -Observed band size:75 kDa(We are unsure as to the identity of these extra bands.) 2. Immunofluorescence staining of GP73 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human GP73 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). |
| Application | WB,ELISA,ICC/IF |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Q8NBJ4-1Met1-Leu401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Golgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human GOLM1 / GP73 Protein |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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