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Anti-GOLPH2/GOLM1 Antibody (6N611)

😃Good
Catalog No. TMAY-02458
Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3

Anti-GOLPH2/GOLM1 Antibody (6N611) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (6N611) can be used in WB,ELISA,ICC/IF.

Anti-GOLPH2/GOLM1 Antibody (6N611)

Anti-GOLPH2/GOLM1 Antibody (6N611)

😃Good
Catalog No. TMAY-02458Alias PSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Anti-GOLPH2/GOLM1 Antibody (6N611) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (6N611) can be used in WB,ELISA,ICC/IF.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-GOLPH2/GOLM1 Antibody (6N611) is a Mouse antibody targeting GOLPH2/GOLM1. Anti-GOLPH2/GOLM1 Antibody (6N611) can be used in WB,ELISA,ICC/IF.
AliasPSEC0257, HEL46, GP73, GOLPH2, golgi membrane protein 1, C9orf155, bA379P1.3
Ig Type
Monoclonal Mouse IgG1
Clone
6N611
Reactivity
Human
Verified Activity
1. Anti-GP73 mouse monoclonal antibody at 1:500 dilution.

-Lane A: 293T Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:45 kDa.

-Observed band size:75 kDa(We are unsure as to the identity of these extra bands.)

2. Immunofluorescence staining of GP73 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human GP73 monoclonal antibody (1:300) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue).
Application
WB,ELISA,ICC/IF
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human GOLM1 / GP73 (rh GOLM1 / GP73; Q8NBJ4-1Met1-Leu401). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundGolgi membrane protein 1, also known as Golgi membrane protein GP73, Golgi phosphoprotein 2, and GOLM1, is a protein that belongs to the GOLM1 / CASC4 family. GOLM1 is widely expressed. It is highly expressed in the colon, prostate, trachea, and stomach. It is expressed at a lower level in testis, muscle, lymphoid tissues, white blood cells, and spleen. It is predominantly expressed by cells of the epithelial lineage. GOLM1 is expressed at a low level in the normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression of GOLM1 does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), GOLM1 is strongly expressed in hepatocyte-derived syncytial giant cells. GOLM1 is constitutively expressed by biliary epithelial cells but not by hepatocytes.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human GOLM1 / GP73 Protein
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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