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LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: LIR5, LILRB4, ILT-3, ILT3, HM18, CD85K) Copy Product InfoSynonyms: LIR5, LILRB4, ILT-3, ILT3, HM18, CD85K
Catalog No. TMPK-00602 Copy Product Info
LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.6 kDa and the accession number is AAH26309.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $91 | 7-10 days | 7-10 days | |
| 10 μg | $148 | 7-10 days | 7-10 days | |
| 20 μg | $245 | 7-10 days | 7-10 days | |
| 50 μg | $483 | 7-10 days | 7-10 days | |
| 100 μg | $813 | 7-10 days | 7-10 days | |
| 200 μg | $1,470 | 7-10 days | 7-10 days | |
| 500 μg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Anti-LILRB4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7.5ng/ml determined by ELISA. |
| Description | LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.6 kDa and the accession number is AAH26309. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | AAH26309 |
| Construction | Gln22-His257 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | LIR5, LILRB4, ILT-3, ILT3, HM18, CD85K |
| Research Background |
| Molecular Weight | 28.6 kDa (predicted). Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | LILRB4/CD85k/ILT3 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight
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