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Anti-Iba1 Antibody (4F442)
😃Good
Catalog No. TMAY-02699
Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (4F442) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (4F442) can be used in WB,ELISA,IP.
Anti-Iba1 Antibody (4F442)
Anti-Iba1 Antibody (4F442)
😃Good
Catalog No. TMAY-02699Alias IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1
Anti-Iba1 Antibody (4F442) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (4F442) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
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Antigen Details
Chemical Properties
| Description | Anti-Iba1 Antibody (4F442) is a Rabbit antibody targeting Iba1. Anti-Iba1 Antibody (4F442) can be used in WB,ELISA,IP. |
| Alias | IRT-1, IRT1, IBA1, allograft inflammatory factor 1, AIF-1 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 4F442 |
| Reactivity | Human |
| Verified Activity | 1. Anti-AIF1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: THP-1 Whole Cell Lysate. -Lane B: HL-60 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:17 kDa. -Observed band size:15 kDa. 2. AIF1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg THP-1 Whole Cell Lysate. -2 µL anti-AIF1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-AIF1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 17 kDa. -Observed band size: 17 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human IBA1 / AIF1 (rh IBA1 / AIF1; TMPY-03337; P55008-1; Met1-Pro147). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | AIF1, also known as IBA1, is an actin-binding protein. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. It is expressed in macrophages and neutrophils. AIF1 enhances membrane ruffling and RAC activation. AIF1 enhances the actin-bundling activity of LCP1. It also enhances lymphocyte migration. AIF1 may play a role in macrophage activation and function. It binds calcium and plays a role in RAC signaling and in phagocytosis. It promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human IBA1 / AIF1 Protein (TMPY-03337) |
| Antigen Species | Human |
| Biology Area | Calcium-binding Proteins and Related Molecules |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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