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Synonyms: VPF, VEGF-A, VEGF, MVCD1, L-VEGF

| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $87 | 7-10 days | 7-10 days | |
| 10 µg | $142 | 7-10 days | 7-10 days | |
| 20 µg | $233 | 7-10 days | 7-10 days | |
| 50 µg | $535 | 7-10 days | 7-10 days |
| Bioactivity | Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50 for this effect is < 8 ng/mL (QC Test). Immobilized Human VEGF110, No Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 0.16 μg/ml determined by ELISA (QC Test). |
| Description | VEGF110 Protein, Human, Recombinant is expressed in HEK293 Cells. The accession number is P15692-9. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | Tag Free |
| Accession Number | P15692-9 |
| Construction | Ala27-Arg136 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | VPF, VEGF-A, VEGF, MVCD1, L-VEGF |
| Research Background | Vascular endothelial growth factor-A (VEGF-A) is a critical angiogenic factor which is mainly secreted from podocytes and epithelial cells in kidney and plays an important role in renal pathophysiology. In recent years, functions of different isoforms of VEGF-A and the new secretion approach via extracellular vesicles (EVs) have been identified. |
| Molecular Weight | 12.7 kDa (predicted); 13 kDa and 17-23 kDa (due to glycosylation) |
| Relative Density. | no data available |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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