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Anti-Pan Influenza A Nucleoprotein Antibody (7E514)
🥰Excellent
Catalog No. TMAY-02897
Anti-Pan Influenza A Nucleoprotein Antibody (7E514) is a Mouse antibody targeting Pan Influenza A Nucleoprotein. Anti-Pan Influenza A Nucleoprotein Antibody (7E514) can be used in WB,ELISA.
Anti-Pan Influenza A Nucleoprotein Antibody (7E514)
Anti-Pan Influenza A Nucleoprotein Antibody (7E514)
🥰Excellent
Catalog No. TMAY-02897
Anti-Pan Influenza A Nucleoprotein Antibody (7E514) is a Mouse antibody targeting Pan Influenza A Nucleoprotein. Anti-Pan Influenza A Nucleoprotein Antibody (7E514) can be used in WB,ELISA.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Pan Influenza A Nucleoprotein Antibody (7E514) is a Mouse antibody targeting Pan Influenza A Nucleoprotein. Anti-Pan Influenza A Nucleoprotein Antibody (7E514) can be used in WB,ELISA. |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 7E514 |
| Verified Activity | 1. Anti-NP mouse monoclonal antibody at 1:1000 dilution. -Sample: Recombinant Protein 10 ng -Lane 1: H1N1 (A/Brevig Mission/1/1918) NP -Lane 2: H1N1 (A/Brisbane/02/2018) NP -Lane 3: H1N1 (A/California/07/2009) NP -Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP -Lane 5: H1N1 (A/Hawaii/70/2019) NP -Lane 6: H1N1 (A/Michigan/45/2015) NP -Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) -Lane 8: H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP -Lane 9: H2N2 (A/Ann Arbor/6/1960) NP -Lane 10: H3N2 (A/Aichi/2/1968) NP -Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP -Lane 12: H3N2 (A/Hong Kong/1/1968) NP -Lane 13: H3N2 (A/Hong Kong/2671/2019) NP -Lane 14: H3N2 (A/Hong Kong/45/2019) NP -Lane 15: H3N2 (A/Hong Kong/4801/2014) NP -Lane 16: H3N2 (A/Kansas/14/2017) NP -Lane 17: H3N2 (A/Switzerland/9715293/2013) NP -Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP -Lane 19: H7N9 (A/Shanghai/2/2013) NP -Lane 20: Influenza B (B/Brisbane/60/2008) NP -Lane 21: Influenza B (B/Colorado/06/2017) NP -Lane 22: Influenza B (B/Florida/4/2006) NP -Lane 23: Influenza B (B/Phuket/3073/2013) NP -Lane 24: Influenza B (B/Washington/02/2019) NP -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. 2. Anti-NP mouse monoclonal antibody at 1 μg/mL. -Sample: Recombinant Protein 10 ng -Lane A: H3N2 (A/Darwin/9/2021)/(A/Darwin/6/2021) NP Protein -Lane B: Influenza B (B/Austria/1359417/2021) NP Protein -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution -Developed using the ECL technique. -Performed under reducing conditions. 3. Anti-Pan Influenza A Nucleoprotein mouse monoclonal antibody in 1μg/mL. -Sample: Recombinant Protein 10 ng -Lane 1: Influenza A H5N1 (A/Texas/37/2024) Nucleoprotein / NP Protein -Lane 2: Influenza A H1N1 (A/Brevig Mission/1/1918) Nucleoprotein / NP Protein -Lane 3: Influenza B (B/Phuket/3073/2013) Nucleoprotein / NP Protein -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution -Developed using the ECL technique. -Performed under reducing conditions. |
| Application | WB,ELISA |
| Recommended Dose | WB: 0.2-1 μg/mL; ELISA: 0.5-1 μg/mL |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A H3N2 (A/Hong Kong/1/1968) Nucleoprotein / NP Protein (40208-V08BP22435.2Met1-Asn498). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Influenza A H3N2 (A/Hong Kong/1/1968) Nucleoprotein / NP Protein (TMPY-00604) |
| Antigen Species | H3N2 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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