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Anti-AURKB Antibody (8S661)
😃Good
Catalog No. TMAH-00097
Alias STK5, STK12, STK-1, STK1, Serine/threonine-protein kinase aurora-B, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase 12, EC 2.7.11.1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora 1, ARK-2, ARK2, AIRK2, AIM-1, AIM1, AIK2
Anti-AURKB Antibody (8S661) is an antibody targeting AURKB. Anti-AURKB Antibody (8S661) can be used in ELISA, WB, IHC, IP.
Anti-AURKB Antibody (8S661)
Anti-AURKB Antibody (8S661)
😃Good
Catalog No. TMAH-00097Alias STK5, STK12, STK-1, STK1, Serine/threonine-protein kinase aurora-B, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase 12, EC 2.7.11.1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora 1, ARK-2, ARK2, AIRK2, AIM-1, AIM1, AIK2
Anti-AURKB Antibody (8S661) is an antibody targeting AURKB. Anti-AURKB Antibody (8S661) can be used in ELISA, WB, IHC, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Chemical Properties
| Description | Anti-AURKB Antibody (8S661) is an antibody targeting AURKB. Anti-AURKB Antibody (8S661) can be used in ELISA, WB, IHC, IP. |
| Alias | STK5, STK12, STK-1, STK1, Serine/threonine-protein kinase aurora-B, Serine/threonine-protein kinase 5, Serine/threonine-protein kinase 12, EC 2.7.11.1, Aurora-related kinase 2, Aurora/IPL1-related kinase 2, Aurora kinase B, Aurora- and IPL1-like midbody-associated protein 1, Aurora 1, ARK-2, ARK2, AIRK2, AIM-1, AIM1, AIK2 |
| Ig Type | Rabbit IgG |
| Clone | 8S661 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate -All lanes: AURKB antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 40, 36, 17, 35 kDa -Observed band size: 40 kDa 2. IHC image of TMAH-00097 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00097 diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunoprecipitating AURKB in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00097 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00097(2µg)+ Hela whole cell lysate(500µg) -Lane 3: Hela whole cell lysate (10µg) |
| Application | ELISA, WB, IHC, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Midbody. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokine... |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Aurora B |
| Antigen Species | Human |
| Gene ID | 9212 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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