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Anti-TNF-alpha Antibody (7S35)

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Catalog No. TMAY-01009

Anti-TNF-alpha Antibody (7S35) is a Mouse antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (7S35) can be used in ELISA,ELISA(Cap),FCM.

Anti-TNF-alpha Antibody (7S35)

Anti-TNF-alpha Antibody (7S35)

😃Good
Catalog No. TMAY-01009
Anti-TNF-alpha Antibody (7S35) is a Mouse antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (7S35) can be used in ELISA,ELISA(Cap),FCM.
Pack SizePriceAvailabilityQuantity
20 μL$947-10 days
100 μL$2927-10 days
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Product Introduction

Bioactivity
Description
Anti-TNF-alpha Antibody (7S35) is a Mouse antibody targeting TNF-alpha. Anti-TNF-alpha Antibody (7S35) can be used in ELISA,ELISA(Cap),FCM.
Ig Type
Mouse IgG1
Clone
7S35
Reactivity
Human
Specificity
Human TNF-alpha
Verified Activity
Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and stimulated lymphocytes. Left panel The transfected cells stained with Purified Mouse anti-TNFa (Bold line hisgram), To demonstrate specificity of staining, the binding of Anti-TNF-alpha Antibody was blocked by the preincubation of the purified antibody with molar excess of recombinant human TNFa (5 μg) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody. Right panel Human PBMC were stimulated for 4 hrs with Leukocyte Activation Cocktail (with BD GolgiPlug™, BD Pharmingen™ Cat. No. 550583), then cells were harvested and treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714).
Application
ELISA
ELISA(Cap)
FCM
Recommended Dose
ELISA: 1:1000-1:2000; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα; TMPY-00936; Val 77-Leu 233; NP_000585.2). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & AntibodyCancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC
Antibody Types Available
2
Antigen Details
Immunogen
Recombinant Protein: Human TNFα protein (TMPY-00936)
Antigen Species
Human
Biology Area
Cancer Drug Targets, Neuroinflammation
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Tumor necrosis factor ligand superfamily member 1Tumor necrosis factorTNF-βTNFβTNF-αTNFαTNF-betaTNF-bTNF-alphaTNF-atnfaLymphotoxin-αLymphotoxinαLymphotoxin-alphaLymphotoxin-a
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