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Anti-MMP-12 Antibody (2O982)

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Catalog No. TMAH-00764
Alias HME

Anti-MMP-12 Antibody (2O982) is an antibody targeting MMP-12. Anti-MMP-12 Antibody (2O982) can be used in ELISA, WB, IHC.

Anti-MMP-12 Antibody (2O982)

Anti-MMP-12 Antibody (2O982)

😃Good
Catalog No. TMAH-00764Alias HME
Anti-MMP-12 Antibody (2O982) is an antibody targeting MMP-12. Anti-MMP-12 Antibody (2O982) can be used in ELISA, WB, IHC.
Pack SizePriceAvailabilityQuantity
50 μL$208 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human
AliasHME
Ig Type
Rabbit IgG
Clone
2O982
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate
-All lanes: MMP12 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 55 kDa
-Observed band size: 54, 45 kDa
2. IHC image of TMAH-00764 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Application
ELISA, WB, IHC
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationSecreted, extracellular space, extracellular matrix.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human MMP12
Antigen Species
Human
Gene ID
4321
Uniprot ID
Biology Area
Cancer, Cardiovascular, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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