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Anti-IGFBP2 Antibody (6E442) is an antibody targeting IGFBP2. Anti-IGFBP2 Antibody (6E442) can be used in ELISA, WB, IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
Alias | mIGFBP-2, IGF-BP53, IGFBP-2, IGFBP2, IBP-2, BP2, AI255832 |
Ig Type | Rabbit IgG |
Clone | 6E442 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate -All lanes: IGFBP2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 35 kDa -Observed band size: 35 kDa 2. IHC image of TMAH-00602 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
Application | ELISA, WB, IHC |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human IGFBP2 |
Antigen Species | Human |
Gene ID | 3485 |
Uniprot ID | |
Biology Area | Cancer, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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