Immobilized Human M-CSF at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8 ng/ml determined by ELISA (QC Test).
Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
Measured in a cell proliferation assay using RAW264.7 cells.The ED50 for this effect is 2.5 - 7.0 ng/mL (QC Test).

M-CSF/CSF1 Protein, Human, Recombinant V2 is expressed in HEK293 Cells. The accession number is P09603-1.

Human

HEK293 Cells

M-CSF,MCSF,CSF-1,colony stimulating factor 1 (macrophage)

Glu33-Asn190

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.