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Anti-PSME1 Antibody (3F619)

😃Good
Catalog No. TMAY-02764
Alias REGα, REGalpha, proteasome (prosome, macropain) activator subunit 1 (PA28 α), proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), PA28α, PA28alpha, PA28A, IFI5111

Anti-PSME1 Antibody (3F619) is a Rabbit antibody targeting PSME1. Anti-PSME1 Antibody (3F619) can be used in WB,ELISA.

Anti-PSME1 Antibody (3F619)

Anti-PSME1 Antibody (3F619)

😃Good
Catalog No. TMAY-02764Alias REGα, REGalpha, proteasome (prosome, macropain) activator subunit 1 (PA28 α), proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), PA28α, PA28alpha, PA28A, IFI5111
Anti-PSME1 Antibody (3F619) is a Rabbit antibody targeting PSME1. Anti-PSME1 Antibody (3F619) can be used in WB,ELISA.
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100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-PSME1 Antibody (3F619) is a Rabbit antibody targeting PSME1. Anti-PSME1 Antibody (3F619) can be used in WB,ELISA.
AliasREGα, REGalpha, proteasome (prosome, macropain) activator subunit 1 (PA28 α), proteasome (prosome, macropain) activator subunit 1 (PA28 alpha), PA28α, PA28alpha, PA28A, IFI5111
Ig Type
Monoclonal Rabbit IgG
Clone
3F619
Reactivity
Human
Verified Activity
Anti-PSME1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HL60 Whole Cell Lysate.

-Lane B: HeLa Whole Cell Lysate.

-Lane C: MCF7 Whole Cell Lysate.

-Lane D: Jurkat Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:29 kDa.

-Observed band size:33 kDa
Application
WB,ELISA
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human PA28A / PSME1 (rh PA28A / PSME1; TMPY-03612; Q06323; Met1-Tyr249).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundPA28A, also known as PSME1, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 2S core and a 19S regulator. The 2S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 1 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME1 gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human PA28A / PSME1 Protein (TMPY-03612)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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