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Anti-CD47 Antibody (8K78)
😃Good
Catalog No. TMAY-03581
Alias OA3, MER6, IAP, CD47 molecule
Anti-CD47 Antibody (8K78) is a Rabbit antibody targeting CD47. Anti-CD47 Antibody (8K78) can be used in WB,ELISA,IP.
Anti-CD47 Antibody (8K78)
Anti-CD47 Antibody (8K78)
😃Good
Catalog No. TMAY-03581Alias OA3, MER6, IAP, CD47 molecule
Anti-CD47 Antibody (8K78) is a Rabbit antibody targeting CD47. Anti-CD47 Antibody (8K78) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD47 Antibody (8K78) is a Rabbit antibody targeting CD47. Anti-CD47 Antibody (8K78) can be used in WB,ELISA,IP. |
| Alias | OA3, MER6, IAP, CD47 molecule |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 8K78 |
| Reactivity | Mouse |
| Verified Activity | 1. Anti-CD47 rabbit polyclonal antibody at 1:500 dilution. -Lane A: Mouse brain tissue lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:35 kDa. 2. mCD47 was immunoprecipitated using: -Lane A:0.5 mg Mouse brain Whole Cell Lysate. -4 µL anti-mCD47 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-mCD47 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size:40 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-5 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD47 (rM CD47; TMPY-04810; Q61735-1; Met1-Lys140). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse CD47 Protein (TMPY-04810) |
| Antigen Species | Mouse |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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