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Anti-Phospho-EIF4E (Ser209) Antibody (4M118)
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Catalog No. TMAH-00894
Alias Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19
Anti-Phospho-EIF4E (Ser209) Antibody (4M118) is an antibody targeting Phospho-EIF4E (Ser209). Anti-Phospho-EIF4E (Ser209) Antibody (4M118) can be used in ELISA, WB, IHC.
Anti-Phospho-EIF4E (Ser209) Antibody (4M118)
Anti-Phospho-EIF4E (Ser209) Antibody (4M118)
😃Good
Catalog No. TMAH-00894Alias Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19
Anti-Phospho-EIF4E (Ser209) Antibody (4M118) is an antibody targeting Phospho-EIF4E (Ser209). Anti-Phospho-EIF4E (Ser209) Antibody (4M118) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | Phospho-EIF4E (S209), p-EIF4E (Ser209), p-EIF4E (S209), mRNA cap binding protein, MGC111573, Messanger RNA Cap Binding Protein eIF 4E, IF4E_HUMAN, Eukaryotic translation initiation factor 4E like 1, Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4 E, EIF4F, EIF4EL1, EIF4E1, EIF4E (p-Ser209), EIF4E (p-S209), eIF 4F 25 kDa subunit, EIF 4F, CBP, AUTS19 |
| Ig Type | Rabbit IgG |
| Clone | 4M118 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in 293 whole cell lysate,HepG2 whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-EIF4E antibody at 1.2μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 25 KDa -Observed band size: 25 KDa 2. IHC image of TMAH-00894 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap. Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis. In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation. May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-EIF4E (S209) |
| Antigen Species | Human |
| Gene ID | 1977 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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