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Anti-S100A5 Antibody (2S480)
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Catalog No. TMAY-01687
Alias S100D9, S100 calcium binding protein A5
Anti-S100A5 Antibody (2S480) is a Mouse antibody targeting S100A5. Anti-S100A5 Antibody (2S480) can be used in ELISA,ICC/IF.
Anti-S100A5 Antibody (2S480)
Anti-S100A5 Antibody (2S480)
😃Good
Catalog No. TMAY-01687Alias S100D9, S100 calcium binding protein A5
Anti-S100A5 Antibody (2S480) is a Mouse antibody targeting S100A5. Anti-S100A5 Antibody (2S480) can be used in ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Chemical Properties
| Description | Anti-S100A5 Antibody (2S480) is a Mouse antibody targeting S100A5. Anti-S100A5 Antibody (2S480) can be used in ELISA,ICC/IF. |
| Alias | S100D9, S100 calcium binding protein A5 |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 2S480 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human S100A5 in A431 or Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human S100A5 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Application | ELISA,ICC/IF |
| Recommended Dose | ELISA: 1:1000-1:2000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A5 (rh S100A5; TMPY-01931; B48219; Met 1-Lys 110). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human S100A5 protein (TMPY-01931) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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