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Anti-Complement C5 Antibody (2J123)
😃Good
Catalog No. TMAH-00129
Alias ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a
Anti-Complement C5 Antibody (2J123) is an antibody targeting Complement C5. Anti-Complement C5 Antibody (2J123) can be used in ELISA, FC.
Anti-Complement C5 Antibody (2J123)
Anti-Complement C5 Antibody (2J123)
😃Good
Catalog No. TMAH-00129Alias ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a
Anti-Complement C5 Antibody (2J123) is an antibody targeting Complement C5. Anti-Complement C5 Antibody (2J123) can be used in ELISA, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Complement C5 Antibody (2J123) is an antibody targeting Complement C5. Anti-Complement C5 Antibody (2J123) can be used in ELISA, FC. |
| Alias | ECLZB, CPAMD4, complement component 5, Complement C5, C5D, C5b, C5a |
| Ig Type | Mouse IgG2a |
| Clone | 2J123 |
| Reactivity | Human |
| Verified Activity | Overlay Peak curve showing Hela cells surface stained with TMAH-00129 (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10, 021 events was performed. |
| Application | ELISA, FC |
| Recommended Dose | FC:1:20-1:500. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Secreted. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Research Background | Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human C5 Protein |
| Antigen Species | Human |
| Gene ID | 727 |
| Uniprot ID | |
| Biology Area | Immunology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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