Anti-APP Antibody (3L659)

Name: Anti-APP Antibody (3L659)
Brand: TargetMol
SKU: TMAH-00073
Rating: 4.5 (1 reviews)

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Catalog No. TMAH-00073

Alias APP695, Amyloid Precursor Protein 695, Amyloid Precursor

Anti-APP Antibody (3L659) is a Mouse antibody targeting APP. Anti-APP Antibody (3L659) can be used in ELISA, WB, IHC, IF.

Anti-APP Antibody (3L659)

😃Good

Catalog No. TMAH-00073Alias APP695, Amyloid Precursor Protein 695, Amyloid Precursor

Anti-APP Antibody (3L659) is a Mouse antibody targeting APP. Anti-APP Antibody (3L659) can be used in ELISA, WB, IHC, IF.

Pack Size	Price	Availability	Quantity
50 μg	$207	7-10 days
100 μg	$348	7-10 days

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Instruction Manual of Detection Antibody

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Product Introduction

Bioactivity

Description	Antibody Type: Mouse Monoclonal Application: ELISA, WB, IHC, IF Reactivity: Human, Mouse
Alias	APP695, Amyloid Precursor Protein 695, Amyloid Precursor
Ig Type	IgG2b
Clone	3L659
Reactivity	Human, Mouse
Verified Activity	1. Western blot -All lanes: Amyloid beta A4 antibody at 1µg/ml -Lane 1: Mouse heart tissue -Lane 2: Mouse kidney tissue -Lane 3: Mouse lung tissue -Secondary: Goat polyclonal to Mouse IgG at 1/15000 dilution -Predicted band size: 30, 35, 72, 80 kDa -Observed band size: 30, 35, 72 kDa 2. Immunohistochemistry of paraffin-embedded human brain tissue using TMAH-00073 at dilution of 1:200 3. Immunofluorescent analysis of Hela cells using TMAH-00073 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) 4. Immunofluorescent analysis of HepG2 cells using TMAH-00073 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Application	ELISA, WB, IHC, IF
Antibody Type	Monoclonal
Host Species	Mouse
Subcellular Localization	Cell membrane; Single-pass type I membrane protein. Membrane; Single-pass type I membrane protein. Perikaryon. Cell projection, growth cone. Membrane, clathrin-coated pit. Early endosome. Cytoplasmic vesicle.; [C83]: Endoplasmic reticulum. Golgi apparatus. Early endosome.; [C99]: Early endosome.; [Soluble APP-beta]: Secreted.; [Amyloid-beta protein 42]: Cell surface.; [Gamma-secretase C-terminal fragment 59]: Nucleus. Cytoplasm.
Construction	Hybridoma Monoclonal Antibody
Purification	Protein G purified
Appearance	Liquid
Formulation	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Purity	>95%
Research Background	Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapes in axons. Involved in copper homeostasis/oxidative stress through copper ion reduction. In vitro, copper-metallated APP induces neuronal death directly or is potentiated through Cu(2+)-mediated low-density lipoprotein oxidation. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV. The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons. Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Bind transient metals such as copper, zinc and iron. In vitro, can reduce Cu(2+) and Fe(3+) to Cu(+) and Fe(2+), respectively. Amyloid-beta protein 42 is a more effective reductant than amyloid-beta protein 40. Amyloid-beta peptides bind to lipoproteins and apolipoproteins E and J in the CSF and to HDL particles in plasma, inhibiting metal-catalyzed oxidation of lipoproteins. APP42-beta may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Interaction with overexpressed HADH2 leads to oxidative stress and neurotoxicity. Also binds GPC1 in lipid rafts. Appicans elicit adhesion of neural cells to the extracellular matrix and may regulate neurite outgrowth in the brain. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis. N-APP binds TNFRSF21 triggering caspase activation and degeneration of both neuronal cell bodies (via caspase-3) and axons (via caspase-6).

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein: Human APP Protein
Antigen Species	Human
Gene ID	351
Uniprot ID	P05067
Biology Area	Neuroscience

Chemical Properties

Stability & Storage

Stability & Storage	Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
Transport	Shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc