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Anti-MAPK1/MAPK3 Antibody (1S651)

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Catalog No. TMAH-00728
Alias PRKM3, PRKM2, PRKM1, p44-MAPK, p44-ERK1, p42-MAPK, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 1, Microtubule-associated protein 2 kinase, MAPK3, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 3, MAP kinase 2, MAP kinase 1, Insulin-stimulated MAP2 kinase, Extracellular signal-regulated kinase 2, Extracellular signal-regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK-1, ERK1, EC 2.7.11.24

Anti-MAPK1/MAPK3 Antibody (1S651) is an antibody targeting MAPK1/MAPK3. Anti-MAPK1/MAPK3 Antibody (1S651) can be used in ELISA, WB, IHC, FC.

Anti-MAPK1/MAPK3 Antibody (1S651)

Anti-MAPK1/MAPK3 Antibody (1S651)

😃Good
Catalog No. TMAH-00728Alias PRKM3, PRKM2, PRKM1, p44-MAPK, p44-ERK1, p42-MAPK, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 1, Microtubule-associated protein 2 kinase, MAPK3, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 3, MAP kinase 2, MAP kinase 1, Insulin-stimulated MAP2 kinase, Extracellular signal-regulated kinase 2, Extracellular signal-regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK-1, ERK1, EC 2.7.11.24
Anti-MAPK1/MAPK3 Antibody (1S651) is an antibody targeting MAPK1/MAPK3. Anti-MAPK1/MAPK3 Antibody (1S651) can be used in ELISA, WB, IHC, FC.
Pack SizePriceAvailabilityQuantity
50 μL$207 7-10 days
100 μL$348 7-10 days
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Product Introduction

Bioactivity
Description
Anti-MAPK1/MAPK3 Antibody (1S651) is an antibody targeting MAPK1/MAPK3. Anti-MAPK1/MAPK3 Antibody (1S651) can be used in ELISA, WB, IHC, FC.
AliasPRKM3, PRKM2, PRKM1, p44-MAPK, p44-ERK1, p42-MAPK, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase 2, Mitogen-activated protein kinase 1, Microtubule-associated protein 2 kinase, MAPK3, MAPK1, MAPK 3, MAPK 2, MAPK 1, MAP kinase isoform p44, MAP kinase isoform p42, MAP kinase 3, MAP kinase 2, MAP kinase 1, Insulin-stimulated MAP2 kinase, Extracellular signal-regulated kinase 2, Extracellular signal-regulated kinase 1, ERT2, ERT1, ERK-2, ERK2, ERK-1, ERK1, EC 2.7.11.24
Ig Type
Rabbit IgG
Clone
1S651
Reactivity
Human, Mouse
Verified Activity
1. Western Blot
-Positive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysate
-All lanes: ERK1/2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 42,44 kDa
-Observed band size: 42,44 kDa
2. IHC image of TMAH-00728 diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.
3. Overlay Peak curve showing Hela cells surface stained with TMAH-00728 (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, IHC, FC
Recommended Dose
WB:1:500-1:2000; IHC:1:50-1:200; FC:1:50-1:200.
Antibody Type
Monoclonal
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human MAPK1/MAPK3
Antigen Species
Human
Uniprot ID
Biology Area
Others
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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