Immobilized Human DLL4, hFc Tag at 1 μg/ml (100 μl/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 16.5 ng/ml determined by ELISA (QC Test).
Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay (Biacore T200).

DLL4 Protein, Human, Recombinant (hFc) V2 is expressed in HEK293 Cells. The accession number is Q9NR61.

Human

HEK293 Cells

δ-like 4 (Drosophila),δ-like 4,hδ2,hdelta2,delta-like 4 (Drosophila),Delta-like 4

Ser27-Pro524

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.