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CD5 Protein, Rhesus macaque, Recombinant (His & Avi)

(Synonyms: T1, LEU1, CD5 molecule, CD5 antigen, CD5) Copy Product Info

Synonyms: T1, LEU1, CD5 molecule, CD5 antigen, CD5

Catalog No. TMPK-00642 Copy Product Info
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma. CD5 Protein, Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 41.7 kDa and the accession number is F6V5Q9.

Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Rhesus macaque CD5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Description
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma. CD5 Protein, Rhesus macaque, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 41.7 kDa and the accession number is F6V5Q9.
Species
Rhesus
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberF6V5Q9
ConstructionArg25-Pro375
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsT1, LEU1, CD5 molecule, CD5 antigen, CD5
Research Background
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Chemical Properties
Molecular Weight41.7 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: CD5 Protein, Rhesus macaque, Recombinant (His & Avi) chemical structure | CD5 Protein, Rhesus macaque, Recombinant (His & Avi) molecular weight