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Anti-EpCAM/TROP1 Antibody (9H261)
😃Good
Catalog No. TMAY-01076
Alias TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5
Anti-EpCAM/TROP1 Antibody (9H261) is a Rabbit antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (9H261) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP.
Anti-EpCAM/TROP1 Antibody (9H261)
Anti-EpCAM/TROP1 Antibody (9H261)
😃Good
Catalog No. TMAY-01076Alias TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5
Anti-EpCAM/TROP1 Antibody (9H261) is a Rabbit antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (9H261) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $94 | 7-10 days | |
| 100 μL | $292 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, ELISA(Det), IHC-P, FCM, ICC/IF, IP Reactivity: Human |
| Alias | TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5 |
| Ig Type | Rabbit IgG |
| Clone | 9H261 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium. 3. Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium. 4. Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium. 5. Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab, then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells. 6. Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution. -Lane A: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:34 kDa. -Observed band size:38 kDa. 7. EpCAM was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -Lane B:0.5 mg A431 Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -Lane D:0.5 mg Caco-2 Whole Cell Lysate -0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-EpCAM rabbit monoclonal antibody, at 1:5000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size: 40 kDa |
| Application | WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IHC-P: 1:1000-1:5000; ICC-IF: 1:250-1:5000; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; TMPY-01300; NP_002345.1; Met 1-Lys 265). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/Biotin/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Human EpCAM protein (TMPY-01300) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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