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Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769)
🥰Excellent
Catalog No. TMAY-02882
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Spike. Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) can be used in ELISA,FCM,ICC/IF.
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769)
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769)
🥰Excellent
Catalog No. TMAY-02882
Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Spike. Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) can be used in ELISA,FCM,ICC/IF.
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Chemical Properties
| Description | Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) is a Chimera antibody targeting SARS-CoV/SARS-CoV-2 Spike. Anti-SARS-CoV/SARS-CoV-2 Spike Antibody (3V769) can be used in ELISA,FCM,ICC/IF. |
| Ig Type | Monoclonal mouse (variable region) / human (kappa / IgG1 constant) chimeric antibody |
| Clone | 3V769 |
| Verified Activity | 1. Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37°C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green). (Validation Experiment) 2. Flow cytometric analysis of SARS-COV-2 Spike overexpressed HEK293 Cells were stained with purified anti-SARS-COV-2 Spike Chimeric MAb, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. (Validation Experiment) 3. Immunofluorescence analysis of SARS-COV-2 Spike (YP_009724390.1, WT) overexpressed HEK293 Cells were stained with purified anti-SARS Spike Chimera Mab,then a FITC-conjugated second step antibody. (Validation Experiment) |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Chimera |
| Construction | It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| Conjucates | Unconjugated |
| Others Formats | Biotin/HRP |
| Antibody Types Available | 3 |
| Immunogen | Recombinant SARS-CoV Spike RBD Protein (TMPY-03224) |
| Antigen Species | SARS |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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