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Anti-Hsp27/HSPB1 Antibody (3Y390)
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Catalog No. TMAY-00670
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (3Y390) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (3Y390) can be used in WB,ELISA,ELISA(Det).
Anti-Hsp27/HSPB1 Antibody (3Y390)
Anti-Hsp27/HSPB1 Antibody (3Y390)
😃Good
Catalog No. TMAY-00670Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (3Y390) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (3Y390) can be used in WB,ELISA,ELISA(Det).
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | 65 € | 7-10 days | |
| 100 μL | 189 € | 7-10 days |
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Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Hsp27/HSPB1 Antibody (3Y390) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (3Y390) can be used in WB,ELISA,ELISA(Det). |
| Alias | SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 3Y390 |
| Reactivity | Human |
| Verified Activity | 1. Anti-HSP27 mouse monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:23 kDa. -Observed band size:26 kDa. 2. HSP27 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -Lane B:0.5 mg Hela Whole Cell Lysate. -2 µL anti-HSP27 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HSP27 mouse monoclonal antibody, at 1:200 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25 kDa. -Observed band size: 25 kDa |
| Application | WB,ELISA,ELISA(Det) |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; TMPY-00823; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Human HSP27 Protein (TMPY-00823) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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