Anti-Hsp27/HSPB1 Antibody (3Y390) is a Mouse antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (3Y390) can be used in ELISA,ELISA(Det),WB.

Mouse IgG2b

3Y390

Human

Human Hsp27/HSPB1
No cross-reactivity in ELISA with
E.coli cell lysate

1. Anti-HSP27 mouse monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:23 kDa.
-Observed band size:26 kDa.
2. HSP27 was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-Lane B:0.5 mg Hela Whole Cell Lysate.
-2 µL anti-HSP27 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-HSP27 mouse monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 25 kDa.
-Observed band size: 25 kDa

WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IP: 4-6 μL/mg of lysate; ELISA(Det): 1:1000-1:10000

Monoclonal

Unconjugated

HRP

2

Recombinant Protein: Human HSP27 Protein (TMPY-00823)

Human

Cancer Drug Targets