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Anti-CDK4 Antibody (6X741)
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Catalog No. TMAH-00253
Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6X741) is an antibody targeting CDK4. Anti-CDK4 Antibody (6X741) can be used in ELISA, WB, IP.
Anti-CDK4 Antibody (6X741)
Anti-CDK4 Antibody (6X741)
😃Good
Catalog No. TMAH-00253Alias PSK-J3, cyclin-dependent kinase 4, CMM3
Anti-CDK4 Antibody (6X741) is an antibody targeting CDK4. Anti-CDK4 Antibody (6X741) can be used in ELISA, WB, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-CDK4 Antibody (6X741) is an antibody targeting CDK4. Anti-CDK4 Antibody (6X741) can be used in ELISA, WB, IP. |
| Alias | PSK-J3, cyclin-dependent kinase 4, CMM3 |
| Ig Type | Rabbit IgG |
| Clone | 6X741 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate -All lanes: CDK4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 21 kDa -Observed band size: 34 kDa 2. Immunoprecipitating CDK4 in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00253 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/1500) -Lane 2:TMAH-00253(2µg)+ Hela whole cell lysate(500µg) -Lane 3: Hela whole cell lysate (10µg) |
| Application | ELISA, WB, IP |
| Recommended Dose | WB:1:500-1:5000; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Nucleus membrane. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CDK4 |
| Antigen Species | Human |
| Gene ID | 1019 |
| Uniprot ID | |
| Biology Area | Isotype/Loading Controls, Epigenetics and Nuclear Signaling, Cancer, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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