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Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902)
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Catalog No. TMAH-00892
Alias Phospho-EIF2AK2 (T446), p-EIF2AK2 (Thr446), p-EIF2AK2 (T446), EIF2AK2 (p-Thr446), EIF2AK2 (p-T446)
Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) is an antibody targeting Phospho-EIF2AK2 (Thr446). Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) can be used in ELISA, WB, IHC, IF.
Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902)
Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902)
😃Good
Catalog No. TMAH-00892Alias Phospho-EIF2AK2 (T446), p-EIF2AK2 (Thr446), p-EIF2AK2 (T446), EIF2AK2 (p-Thr446), EIF2AK2 (p-T446)
Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) is an antibody targeting Phospho-EIF2AK2 (Thr446). Anti-Phospho-EIF2AK2 (Thr446) Antibody (4K902) can be used in ELISA, WB, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IF Reactivity: Human |
| Alias | Phospho-EIF2AK2 (T446), p-EIF2AK2 (Thr446), p-EIF2AK2 (T446), EIF2AK2 (p-Thr446), EIF2AK2 (p-T446) |
| Ig Type | Rabbit IgG |
| Clone | 4K902 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-EIF2AK2 antibody at 1.25μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62 KDa -Observed band size: 62 KDa 2. IHC image of TMAH-00892 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunofluorescence staining of Hela cells(treated with 50mM Calyculin A for 30min) with TMAH-00892 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IHC, IF |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Cytoplasm, perinuclear region. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | IFN-induced dsRNA-dependent serine/threonine-protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) and plays a key role in the innate immune response to viral infection. Inhibits viral replication via the integrated stress response (ISR): EIF2S1/eIF-2-alpha phosphorylation in response to viral infection converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, resulting to a shutdown of cellular and viral protein synthesis, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activator ATF4. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation: phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-EIF2AK2 (T446) |
| Antigen Species | Human |
| Gene ID | 5610 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Keywords
Tyrosine-protein kinase EIF2AK2Protein kinase RNA-activated (PKR;Protein kinase R)PRKRPKRpEIF2AK2 (Thr446)pEIF2AK2 (T446)p68 kinaseP1/eIF-2A protein kinaseInterferon-inducible RNA-dependent protein kinaseInterferon-induced, double-stranded RNA-activated protein kinaseEukaryotic translation initiation factor 2-α kinase 2 (eIF-2A protein kinase 2)Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2)Eukaryotic translation initiation factor 2-a kinase 2 (eIF-2A protein kinase 2)EIF2AK2 (pThr446)EIF2AK2 (pT446)EIF2AK2 (phospho-Thr446)EIF2AK2 (phospho-T446)EIF2AK2EIF2AK 2eIF2A protein kinase2eIF-2A protein kinase 2double-stranded RNA-activated protein kinase
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