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Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) (Synonyms: Tri-methyl-Histone H4 (K20), Tri-Me-Histone H4 (...)

Catalog No. TMAH-01213 Copy Product Info
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Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA,ICC,WB.

Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998)

Copy Product Info
🥰Excellent
Catalog No. TMAH-01213
Synonyms Tri-methyl-Histone H4 (K20), Tri-Me-Histone H4 (Lys20), Tri-Me-Histone H4 (K20), TriMe-H4K20, Histone H4K20-trimethylated, H4K20me3

Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA,ICC,WB.

Anti-Tri-methyl-Histone H4
(Lys20) Antibody
(4H998)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$208-In Stock
100 μL$3497-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Appearance:Liquid
Color:Transparent
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Product Introduction

Bioactivity
Description
Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA,ICC,WB.
SynonymsTri-methyl-Histone H4 (K20), Tri-Me-Histone H4 (Lys20), Tri-Me-Histone H4 (K20), TriMe-H4K20, Histone H4K20-trimethylated, H4K20me3
Ig Type
Rabbit IgG
Clone
4H998
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in MCF-7 whole cell lysate, Mouse brain tissue,Mouse kidney tissue
-All lanes Tri-methyl-Histone H4 (K20) antibody at 2.15μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 11 KDa
-Observed band size: 11 KDa
2. Immunocytochemistry analysis of TMAH-01213 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
Recommended Dose
WB:1:500-1:5000; ICC:1:50-1:300.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Chromosome.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide
Antigen Species
Human
Gene Name
HIST1H4A
Gene ID
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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