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Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA,ICC,WB.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
| Description | Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (Lys20). Anti-Tri-methyl-Histone H4 (Lys20) Antibody (4H998) can be used in ELISA,ICC,WB. |
| Synonyms | Tri-methyl-Histone H4 (K20), Tri-Me-Histone H4 (Lys20), Tri-Me-Histone H4 (K20), TriMe-H4K20, Histone H4K20-trimethylated, H4K20me3 |
| Ig Type | Rabbit IgG |
| Clone | 4H998 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in MCF-7 whole cell lysate, Mouse brain tissue,Mouse kidney tissue -All lanes Tri-methyl-Histone H4 (K20) antibody at 2.15μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 11 KDa -Observed band size: 11 KDa 2. Immunocytochemistry analysis of TMAH-01213 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | |
| Recommended Dose | WB:1:500-1:5000; ICC:1:50-1:300. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Chromosome. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide |
| Antigen Species | Human |
| Gene Name | HIST1H4A |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |

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