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Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998)

🥰Excellent
Catalog No. TMAH-01213

Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (K20). Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) can be used in ELISA, WB, ICC.

Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998)

Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998)

🥰Excellent
Catalog No. TMAH-01213
Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (K20). Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) can be used in ELISA, WB, ICC.
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Product Introduction

Bioactivity
Description
Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) is an antibody targeting Tri-methyl-Histone H4 (K20). Anti-Tri-methyl-Histone H4 (K20) Antibody (4H998) can be used in ELISA, WB, ICC.
Ig Type
Rabbit IgG
Clone
4H998
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in MCF-7 whole cell lysate, Mouse brain tissue,Mouse kidney tissue
-All lanes Tri-methyl-Histone H4 (K20) antibody at 2.15μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 11 KDa
-Observed band size: 11 KDa
2. Immunocytochemistry analysis of TMAH-01213 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Application
ELISA, WB, ICC
Recommended Dose
WB:1:500-1:5000; ICC:1:50-1:300.
Antibody Type
Monoclonal
Subcellular LocalizationNucleus. Chromosome.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundCore component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthesized peptide
Antigen Species
Human
Gene ID
121504
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Histone H4HIST4H4HIST4H 4HIST2H4BHIST2H4AHIST2H4HIST2H 4HIST1H4LHIST1H4KHIST1H4JHIST1H4IHIST1H4HHIST1H4FHIST1H4EHIST1H4DHIST1H4CHIST1H4BHIST1H4AH4FOH4FNH4FMH4FKH4FJH4FIH4FHH4FGH4FEH4FDH4FCH4FBH4FAH4F2H4F 2H4C9H4C8H4C6H4C5H4C4H4C3H4C2H4C16H4C15H4C14H4C13H4C12H4C11H4C1H4C 9H4C 8H4C 6H4C 5H4C 4H4C 3H4C 2H4C 16H4C 15H4C 14H4C 13H4C 12H4C 11H4C 1H4-16H416H4/OH4/NH4/MH4/KH4/JH4/IH4/HH4/GH4/EH4/DH4/CH4/BH4/A
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