IgE Protein, Human, Recombinant (His & Avi)

Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.

Pack Size	Availability	Price/USD
100 μg	In stock	$ 581.00
500 μg	5 days	$ 2,330.00
1 mg	5 days	$ 3,880.00

Pack Size

Availability

Price/USD

Quantity

100 μg

In stock

$ 581.00

500 μg

5 days

$ 2,330.00

1 mg

5 days

$ 3,880.00

Biological Information

Immobilized Human IgE, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-IgE Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.

Description

Species	Human
Expression System	HEK293
Tag	C-His-Avi
Accession Number	P01854
Synonyms	Ig ε chain C region, IgE, Ig epsilon chain C region
Construction	Recombinant Human IgE Protein is expressed from HEK293 with His and Avi tag at the C-Terminus. It contains Cys209-Lys428.[Accession \|P01854]
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Immunoglobulin E (IgE) is well known for its role in allergic disease, the manifestations of which are mediated through its two Fc receptors, FcεRI and CD23 (FcεRII). IgE and its interactions with these receptors are therefore potential targets for therapeutic intervention, and exciting progress has been made in this direction. Furthermore, recent structural studies of IgE-Fc, the two receptors, and of their complexes, have revealed a remarkable degree of plasticity at the IgE-CD23 interface and an even more remarkable degree of dynamic flexibility within the IgE molecule.