DNAM-1/CD226 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00229 Copy Product Info

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. DNAM-1/CD226 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.9 kDa and the accession number is Q15762.

DNAM-1/CD226 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00229

Copy Product Info

DNAM-1/CD226 Protein, Human, Recombinant (His & Avi)

Pack Size	Price	USA Stock	Global Stock
5 μg	$41	7-10 days	7-10 days
10 μg	$62	7-10 days	7-10 days
20 μg	$98	7-10 days	7-10 days
50 μg	$188	7-10 days	7-10 days
100 μg	$313	7-10 days	7-10 days
200 μg	$568	7-10 days	7-10 days
500 μg	$1,260	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	1. Immobilized Human DNAM-1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.24μg/ml determined by ELISA (QC Test). 2. Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay.
Description	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. DNAM-1/CD226 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.9 kDa and the accession number is Q15762.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q15762
Synonyms	TLiSA1,PTA1,DNAM1,CD226 molecule,CD226
Construction	Glu19-Asn247
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Molecular Weight	28.9 kDa (predicted). Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.