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Anti-BCL6 Polyclonal Antibody is a Rabbit antibody targeting BCL6. Anti-BCL6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days |
Description | Anti-BCL6 Polyclonal Antibody is a Rabbit antibody targeting BCL6. Anti-BCL6 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Alias | ZNF51, ZBTB27, LAZ3, BCL6A, BCL5, B-cell CLL/lymphoma 6 |
Ig Type | IgG |
Reactivity | Human,Mouse |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (TMAB-00201) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (Bcl-6) Polyclonal Antibody, Unconjugated (TMAB-00201) at 1:25 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. 25 μg total protein per Lane of various lysates probed with Bcl-6 polyclonal antibody, unconjugated (TMAB-00201) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Nucleus (By similarity). |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]. |
Immunogen | KLH conjugated synthetic peptide: human Bcl-6 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Transcription factors,Proto-oncogenes,Zinc Finger |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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