CD19 Protein, Human, Recombinant (His & Avi), Biotinylated

Immobilized Biotinylated Human CD19, His-Avi Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Coltuximab, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
Immobilized Biotinylated Human CD19, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for FMC63, mFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
Anti-CD19 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human CD19, His Tag with an affinity constant of 0.64 nM as determined in SPR assay (Biacore T200).
Loaded Coltuximab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 4.53 nM as determined in BLI assay (Gator® Prime).
Loaded Inebilizumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of 11.00 nM as determined in BLI assay (Gator® Prime).
Loaded Denintuzumab on ProA-Biosensor can bind Biotinylated Human CD19, His-Avi Tag with an affinity constant of4.70 nM as determined in BLI assay (Gator® Prime).

CD19 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 33.37 kDa and the accession number is P15391-1.

Human

HEK293 Cells

≥ 95 % as determined by SDS-PAGE.

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy