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Anti-UBE2W Antibody (7C854)
😃Good
Catalog No. TMAY-02413
Alias ubiquitin-conjugating enzyme E2W (putative), UBC-16, UBC16
Anti-UBE2W Antibody (7C854) is a Mouse antibody targeting UBE2W. Anti-UBE2W Antibody (7C854) can be used in ELISA.
Anti-UBE2W Antibody (7C854)
Anti-UBE2W Antibody (7C854)
😃Good
Catalog No. TMAY-02413Alias ubiquitin-conjugating enzyme E2W (putative), UBC-16, UBC16
Anti-UBE2W Antibody (7C854) is a Mouse antibody targeting UBE2W. Anti-UBE2W Antibody (7C854) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $199 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-UBE2W Antibody (7C854) is a Mouse antibody targeting UBE2W. Anti-UBE2W Antibody (7C854) can be used in ELISA. |
| Alias | ubiquitin-conjugating enzyme E2W (putative), UBC-16, UBC16 |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 7C854 |
| Reactivity | Human |
| Application | ELISA |
| Recommended Dose | ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE2W (rh UBE2W; TMPY-02642; Q96B02-12; Met1-Cys151). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Ubiquitin-conjugating enzymes, also known as UBE2W, E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step of protein ubiquitination. The modification of protein with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2W is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. It accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. It also catalyzes monoubiquitination and "Lys-11"-linked polyubiquitination. UBE2W is also considered to regulate FANCD2 monoubiquitination. UBE2W exhibits ubiquitin conjugating enzyme activity and catalyzes the monoubiquitination of PHD domain of Fanconi anemia complementation group L (FANCL). Over-expression of UBE2W in cells promotes the monoubiquitination of FANCD2 and down-regulated UBE2W markedly reduces the UV irradiation-induced but not MMC-induced FANCD2 monoubiquitination. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human UBE2W protein (TMPY-02642) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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