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Anti-Phospho-MAPT (Ser202) Antibody (2U827) is an antibody targeting Phospho-MAPT (Ser202). Anti-Phospho-MAPT (Ser202) Antibody (2U827) can be used in ELISA, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $207 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, FCM Reactivity: Human |
Alias | TAU and MAPT, p-MAPT (Ser202), p-MAPT (S202), Phospho-MAPT (S202), Paired helical filament-tau, Neurofibrillary tangle protein, MTBT1, Microtubule-associated protein tau, MAPTL, MAPT (p-Ser202), MAPT (p-S202) |
Ig Type | Rabbit IgG |
Clone | 2U827 |
Reactivity | Human |
Verified Activity | Overlay Peak curve showing SH-SY5Y cells stained with TMAH-00921 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, FCM |
Recommended Dose | FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human MAPT |
Antigen Species | Human |
Gene ID | 4137 |
Uniprot ID | |
Biology Area | Neuroscience, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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