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Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $92 | 7-10 days | |
100 μL | $270 | 7-10 days |
Description | Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP. |
Alias | SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
Ig Type | Monoclonal Rabbit IgG |
Clone | 7V28 |
Reactivity | Human |
Verified Activity | 1. HSP27 was immunoprecipitated using: -Lane A:0.5 mg Caco-2 Whole Cell Lysate. -2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HSP27 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25 kDa. -Observed band size: 25 kDa. 2. Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:23 kDa. -Observed band size:26 kDa |
Application | WB,ELISA,ELISA(Cap),IP |
Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; TMPY-00823; NP_001532.1; Met 1-Lys 205) . |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Conjucates | Unconjugated |
Immunogen | Recombinant Human HSP27 protein (TMPY-00823) |
Antigen Species | Human |
Biology Area | Cancer Drug Targets |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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