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Anti-Hsp27/HSPB1 Antibody (7V28)

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Catalog No. TMAY-00672
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F

Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.

Anti-Hsp27/HSPB1 Antibody (7V28)

Anti-Hsp27/HSPB1 Antibody (7V28)

😃Good
Catalog No. TMAY-00672Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.
Pack SizePriceAvailabilityQuantity
20 μL$92 7-10 days
100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.
AliasSRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Ig Type
Monoclonal Rabbit IgG
Clone
7V28
Reactivity
Human
Verified Activity
1. HSP27 was immunoprecipitated using:

-Lane A:0.5 mg Caco-2 Whole Cell Lysate.

-2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-HSP27 rabbit monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 25 kDa.

-Observed band size: 25 kDa.

2. Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:23 kDa.

-Observed band size:26 kDa
Application
WB,ELISA,ELISA(Cap),IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; TMPY-00823; NP_001532.1; Met 1-Lys 205) .
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human HSP27 protein (TMPY-00823)
Antigen Species
Human
Biology Area
Cancer Drug Targets
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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