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Anti-Hsp27/HSPB1 Antibody (7V28)
😃Good
Catalog No. TMAY-00672
Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.
Anti-Hsp27/HSPB1 Antibody (7V28)
Anti-Hsp27/HSPB1 Antibody (7V28)
😃Good
Catalog No. TMAY-00672Alias SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F
Anti-Hsp27/HSPB1 Antibody (7V28) is a Rabbit antibody targeting Hsp27/HSPB1. Anti-Hsp27/HSPB1 Antibody (7V28) can be used in WB,ELISA,ELISA(Cap),IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, ELISA(Cap), IP Reactivity: Human |
| Alias | SRP27, HSPB1, HSP28, Hsp27, HSP 27, HMN2B, CMT2F |
| Ig Type | Rabbit IgG |
| Clone | 7V28 |
| Reactivity | Human |
| Verified Activity | 1. HSP27 was immunoprecipitated using: -Lane A:0.5 mg Caco-2 Whole Cell Lysate. -2 µL anti-HSP27 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-HSP27 rabbit monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 25 kDa. -Observed band size: 25 kDa. 2. Anti-HSP27 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:23 kDa. -Observed band size:26 kDa |
| Application | WB,ELISA,ELISA(Cap),IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; TMPY-00823; NP_001532.1; Met 1-Lys 205) . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human HSP27 protein (TMPY-00823) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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