Anti-PTBP1 Antibody (9D226) is an antibody targeting PTBP1. Anti-PTBP1 Antibody (9D226) can be used in ELISA,IHC,WB.

Rabbit IgG

9D226

Human

1. Western Blot
-Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate
-All lanes: PTBP1 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 58, 60, 60 kDa
-Observed band size: 58 kDa
2. IHC image of TMAH-01012 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-01012 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

WB:1:500-1:5000; IHC:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human PTBP1

Human

PTBP1

Epigenetics and Nuclear Signaling