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Anti-PTBP1 Antibody (9D226)
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Catalog No. TMAH-01012
Alias PTBP 1, PTB, Polypyrimidine tract-binding protein 1, hnRNP I, Heterogeneous nuclear ribonucleoprotein I, 57 kDa RNA-binding protein PPTB-1
Anti-PTBP1 Antibody (9D226) is an antibody targeting PTBP1. Anti-PTBP1 Antibody (9D226) can be used in ELISA, WB, IHC.
Anti-PTBP1 Antibody (9D226)
Anti-PTBP1 Antibody (9D226)
😃Good
Catalog No. TMAH-01012Alias PTBP 1, PTB, Polypyrimidine tract-binding protein 1, hnRNP I, Heterogeneous nuclear ribonucleoprotein I, 57 kDa RNA-binding protein PPTB-1
Anti-PTBP1 Antibody (9D226) is an antibody targeting PTBP1. Anti-PTBP1 Antibody (9D226) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | PTBP 1, PTB, Polypyrimidine tract-binding protein 1, hnRNP I, Heterogeneous nuclear ribonucleoprotein I, 57 kDa RNA-binding protein PPTB-1 |
| Ig Type | Rabbit IgG |
| Clone | 9D226 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate -All lanes: PTBP1 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 58, 60, 60 kDa -Observed band size: 58 kDa 2. IHC image of TMAH-01012 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-01012 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human PTBP1 |
| Antigen Species | Human |
| Gene ID | 5725 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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