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IgE Protein, Mouse, Recombinant (His & Avi), Biotinylated
(Synonyms: IgE, Ig ε chain C region, Ig epsilon chain C region) Copy Product InfoSynonyms: IgE, Ig ε chain C region, Ig epsilon chain C region
Catalog No. TMPK-01719 Copy Product Info
IgE Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P06336.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human Fc epsilon RI alpha, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Mouse IgE, His Avi Tag with the EC50 of 42.0 ng/ml determined by ELISA (QC Test). Human Fc epsilon RI alpha, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse IgE, His-Avi Tag with an affinity constant of 0.42 nM as determined in SPR assay (Biacore T200). |
| Description | IgE Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P06336. |
| Species | Mouse |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P06336 |
| Construction | Asp198-Ser421 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | IgE, Ig ε chain C region, Ig epsilon chain C region |
| Research Background |
| Molecular Weight | 28.2 kDa (Predicted); 35-45 kDa (Reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: IgE Protein, Mouse, Recombinant (His & Avi), Biotinylated chemical structure | IgE Protein, Mouse, Recombinant (His & Avi), Biotinylated molecular weight
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