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P-Selectin Protein, Human, Recombinant (Avi & His), Biotinylated
(Synonyms: SELP, P-Selectin, PSEL, PADGEM, LECAM3, GRMP, GMP140, FLJ45155, CD62P, CD62) Copy Product InfoSynonyms: SELP, P-Selectin, PSEL, PADGEM, LECAM3, GRMP, GMP140, FLJ45155, CD62P, CD62
Catalog No. TMPK-00830 Copy Product Info
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $91 | - | In Stock | |
| 10 µg | $148 | - | In Stock | |
| 20 µg | $245 | - | In Stock | |
| 50 µg | $483 | - | In Stock | |
| 100 µg | $813 | - | In Stock | |
| 200 µg | $1,470 | 7-10 days | 7-10 days | |
| 500 µg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Solution
Color:Transparent
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human P-Selectin, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. |
| Description | Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P16109 |
| Construction | Trp42-Ala771 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% saccharose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | SELP, P-Selectin, PSEL, PADGEM, LECAM3, GRMP, GMP140, FLJ45155, CD62P, CD62 |
| Research Background | Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
| Molecular Weight | 82.9 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: P-Selectin Protein, Human, Recombinant (Avi & His), Biotinylated chemical structure | P-Selectin Protein, Human, Recombinant (Avi & His), Biotinylated molecular weight
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