Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 814.00 | |
500 μg | 5 days | $ 3,250.00 | |
1 mg | 5 days | $ 5,440.00 |
Description | Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P16109 |
Synonyms | FLJ45155, CD62P, GRMP, CD62, P-Selectin, PSEL, GMP140, LECAM3, SELP, PADGEM |
Construction | Recombinant Biotinylated Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Trp42-Ala771.[Accession |P16109] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% saccharose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. |
bottom
Please read the User Guide of Recombinant Proteins for more specific information.
P-Selectin Protein, Human, Recombinant (Avi & His), Biotinylated FLJ45155 CD62P GMP 140 GMP-140 FLJ-45155 GRMP CD62 LECAM 3 P-Selectin PSEL CD 62 GMP140 LECAM3 CD-62 SELP FLJ 45155 LECAM-3 PADGEM recombinant recombinant-proteins proteins protein