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Anti-Phospho-MYC (Ser62) Antibody (5C154) is an antibody targeting Phospho-MYC (Ser62). Anti-Phospho-MYC (Ser62) Antibody (5C154) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | p-MYC (Ser62), p-MYC (S62), Phospho-MYC (S62), MYC (p-Ser62), MYC (p-S62), BHLHE39 |
Ig Type | Rabbit IgG |
Clone | 5C154 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate -All lanes Phospho-MYC antibody at 1.4μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 57 KDa -Observed band size: 57 KDa 2. Immunofluorescence staining of Hela cells with TMAH-00932 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human Phospho-MYC (S62) |
Antigen Species | Human |
Gene ID | 4609 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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