Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC, IF

Reactivity: Human

Rabbit IgG

1S803

Human

1. Western Blot
-Positive WB detected in: U251 whole cell lysate, MCF-7 whole cell lysate, HEK293 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate
-All lanes: PIN4 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 14, 17, 15 kDa
-Observed band size: 12-17 kDa
2. IHC image of TMAH-00981 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. Immunofluorescence staining of MCF-7 cell with TMAH-00981 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 598-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, WB, IHC, IF

WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human PIN4

Human

5303

Epigenetics and Nuclear Signaling, Metabolism, Signal transduction