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Anti-PRKAR1A Antibody (6V492) is an antibody targeting PRKAR1A. Anti-PRKAR1A Antibody (6V492) can be used in ELISA, WB, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
Alias | TSE1, protein kinase, cAMP-dependent, regulatory, type I, α, protein kinase, cAMP-dependent, regulatory, type I, alpha, PRKAR1, PPNAD1, PKR1, CNC1, CNC, CAR, ADOHR, ACRDYS1 |
Ig Type | Rabbit IgG |
Clone | 6V492 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: U87 whole cell lysate -All lanes: PRKAR1A antibody at 1.1μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 43, 39 KDa -Observed band size: 43 KDa 2. Immunofluorescence staining of Hela cells with TMAH-01002 at 1:36, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, WB, IF |
Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cell membrane. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human PRKAR1A |
Antigen Species | Human |
Gene ID | 5573 |
Uniprot ID | |
Biology Area | Signal Transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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