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Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738)
🥰Excellent
Catalog No. TMAY-02953
Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) can be used in WB,ELISA,ELISA(Cap).
Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738)
Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738)
🥰Excellent
Catalog No. TMAY-02953
Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) can be used in WB,ELISA,ELISA(Cap).
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Chemical Properties
| Description | Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) is a Mouse antibody targeting SARS-CoV-2 Nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid Antibody (8R738) can be used in WB,ELISA,ELISA(Cap). |
| Ig Type | Monoclonal Mouse IgG1 |
| Clone | 8R738 |
| Verified Activity | Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid mouse monoclonal antibody at 1:2000 dilution. -Lane A: SARS-COV-2 Nucleocapsid (YP_009724397.2, WT) overexpressed HEK293 Whole Cell Lysate. -Lane B: HEK293 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. (Validation Experiment) |
| Application | WB,ELISA,ELISA(Cap) |
| Recommended Dose | WB: 1:2000-1:5000; ELISA: 1:1000-1:2000; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, SARS-CoV-2 (2019-nCoV) Nucleocapsid (TMPY-05664; YP_009724397.2(335Gly/Ala); Met1-Ala419). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (TMPY-05664) |
| Antigen Species | SARS-CoV-2 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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